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protogel83. Pour Stacking Gel Use ProtoGel Stacking Buffer to make 10 mL of a 4% stacking gel as follows: ProtoGel: 1.33 mL ProtoGel Stacking Buffer: 2.50 mLProtocol Twenty Minute Casting. ProtoGel Quick-Cast contains the monomers and buffer components to produce a 12% gel. Measure out the volume of